Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

Figure 9

Influence of altered gravity during sounding rocket flight on ABC transporter genes. ABCC1, ABCC4, ABCD4, ABCF2, and TAP2 RNA expression levels after launch and acceleration (BL, dark gray) and μg (black) conditions of TEXUS-49. Hardware ground controls (H/W, striped) are shown for each experimental group. RNA levels are depicted as fluorescence intensities. Only ABCC1 expression is stable over all g conditions. ABCC4, ABCD4, ABCF2, and TAP2 display μg sensitivity compared to BL and to H/W in the case of ABCF2 and TAP2. TAP2 also shows vibration sensitivity comparing BL to H/W. Mean values of at least three measurements with standard deviations are shown. , , and .