Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 363575, 20 pages
http://dx.doi.org/10.1155/2015/363575
Research Article

Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

1Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, Switzerland
2Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany
3Study Group “Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen” (MARS), Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany
4Arrows Biomedical Deutschland GmbH, Center for Nanotechnology at the Westfälische Wilhelms-Universität Münster, Heisenbergstraße 11, 48149 Münster, Germany
5German Aerospace Center, Space Agency, Königswinterer Straße 522-524, 53227 Bonn, Germany
6KEK GmbH, Kemberger Straße 5, 06905 Bad Schmiedeberg, Germany
7University of Applied Science Jena, Carl-Zeiss-Promenade 2, 07745 Jena, Germany
8Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Winterthurerstraße 190, 8057 Zurich, Switzerland

Received 14 May 2014; Accepted 4 September 2014

Academic Editor: Jack J. W. A. Van Loon

Copyright © 2015 Cora S. Thiel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1 summarizes the differential gene expression analysis of the potential reference genes presented in our study. Fluorescence, mean fluorescence and standard deviation values are given for the individual gene probes at different g-conditions measured during the 19th DLR Parabolic flight campaign as well as for the TEXUS-49 sounding rocket mission. Gene expression levels were compared between the different g-conditions for those genes that are represented by at least 3 probes and p-values were calculated. Significant p-values below 0,05 are displayed in red.

Supplementary Table 2 shows the analysis of the ABC transporter genes. Fluorescence, mean fluorescence and standard deviation values are given for the individual gene probes at different g-conditions measured during the 19th DLR Parabolic flight campaign as well as for the TEXUS-49 sounding rocket mission. Gene expression levels were compared between the different gconditions for those genes that are represented by at least 3 probes and p-values were calculated. Significant p-values below 0,05 are displayed in red.

Supplementary Table 3 displays the analyzed tRNA related genes. Fluorescence, mean fluorescence and standard deviation values are given for the individual gene probes at different g-conditions measured during the 19th DLR Parabolic flight campaign as well as for the TEXUS-49 sounding rocket mission. Gene expression levels were compared between the different g-conditions for those genes that are represented by at least 3 probes and p-values were calculated. Significant p-values below 0,05 are displayed in red.

  1. Supplementary Tables