miR-125b Suppresses Proliferation and Invasion by Targeting MCL1 in Gastric Cancer

Figure 2

MCL1 is a target of miR-125b in breast cancer cells. (a) Schematic of the predicted miR-125b site in the 3′UTR of MCL1 mRNA, which is broadly conserved among vertebrates. (b) miR-125b expression was detected in MGC-803 cells transfected with either miR-125b mimics or the miR-ctr control by qRT-PCR. The error bars represent the standard deviations (SD) from triplicates of one representative experiment. . (c) Luciferase reporter assays were performed after transfection of the indicated pMIR-Report plasmids, a Renilla transfection control plasmid, and with either miR-34a or the relevant scrambled controls. The data shown are the means ± SD of three replicates and are representative of three independent experiments. . (d) MCL1 mRNA and protein expression levels were much lower in MGC-803 cells transfected with miR-125b mimics compared to cells transfected with miR-ctr. The data shown are the means ± SD of three replicates and are representative of three independent experiments. .

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