miR-125b Suppresses Proliferation and Invasion by Targeting MCL1 in Gastric Cancer

Figure 4

miR-125b inhibited the gastric cancer invasion ability in vitro by targeting Rhoc. (a) MGC-803 were transfected with 5-FU, miR-125b mimics, si-MCL1, or combinations of the reagents. MTT assays were performed in MGC-803 cells 48 h after treatment. miR-125b, miR-ctr, si-MCL1, or si-ctr was transiently transfected at a concentration of 40 nM. The work concentration of 5-FU was 10 ng/mL. All data are shown as the mean ± SEM, , . (b) MGC-803 were transfected with 5-FU, miR-125b mimics, si-MCL1, or combinations of the reagents. Invasion assays were evaluated by the Transwell assay. (c) MGC-803 were transfected with 5-FU, miR-125b mimics, si-MCL1, or combinations of the reagents. The apoptotic cells were evaluated by Annexin V-FITC and propidium iodine staining and analyzed with FACS.

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