Complete Nucleotide Sequence Analysis of the Norovirus GII.4 Sydney Variant in South Korea

Table 1

Information of primers used in this study.

Region	Primers	Sequence (5′-3′)	Polarity	Position^a	Reference
ORF1-ORF2	GII-F1M	GGG AGG GCG ATC GCA ATC T	+	5049–5067
	GII-R1M	CCR CCI GCA TRI CCR TTR TAC AT	−	5367–5389
ORF1	ORF1-1F	GTG AAT GAA GAT GGC GTC TA	+	1–20	[23]
	ORF1-1R	AGT CTT GGT AGG GCC TAA AG	−	697–716
	ORF1-2F	GGC TAA GCA GGA GAA TGA TTC A	+	660–681	In this study
	ORF1-2R	GAG AGT TGA TTG TGC CCA CA	−	1375–1394
	ORF1-3F	CCA AGT CTG CTT CAC CTG AC	+	1353–1372	[23]
	ORF1-3R	GGG TGT TTC CGT TCT TGT C	−	1976–1994
	ORF1-4F	ACT GTC ATT GGC TCC ACA G	+	1948–1966	In this study
	ORF1-4R	TGT GTG CTT CTT GCC ACG	−	2657–2674
	ORF1-5F	GAC GAC ATC AAA ACT GAG GGC	+	2612–2632
	ORF1-5R	TCT TGA TGA GCA GTG TGG C	−	3275–3293
	ORF1-6F	GGC ATG ATC TTG GAA GAA GG	+	3236–3255	[23]
	ORF1-6R	ATG CTT GCG CGA ATG ACC	−	3882–3899
	ORF1-7F	GGC TGC CAA GAA AAC CAT C	+	3823–3841
	ORF1-7R	ACC TCA GAA AGT GCA CAG AG	−	4523–4542
	ORF1-8F	CCA ATG GAA TTC CAT CGC CC	+	4489–4508
	ORF1-8R	CGA CGC CAT CTT CAT TCA CA	−	5080–5099
ORF2	ORF2-F1	AAG AGC CAA TGT TCA GAT GG	+	5004–5023	[22]
	ORF2-R1	CTC TGA AGG TGC AGA TGT T	−	5928–5946
	ORF2-F2	AAC ATC TGC ACC TTC AGA G	+	5928–5946
	ORF2-R2	GAA GCC TGT TGT AGA TTG CT	−	6854–6873
ORF3	ORF 3F	ATG GCT GGA GCT TTC TTT GCT	+	6704–6724
	ORF 3R	AAA GAC ACT AAA GAA AGG AAA GAT	−	7532–7555

Positions are indicated relative to the Lordsdale strain (GenBank accession number X86557).