Research Article
Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica
Figure 5
E. histolytica trophozoites bind and internalize B-holo-Lf with high affinity; however, virulent amoebae show higher and number of EhBholoLfbp than nonvirulent amoebae. ((a), (c)) Amoebae (5 × 105 cells) were synchronized and incubated with several concentrations of FITC-B-holo-Lf (1–12,000 nM) at 6 or 37°C for 30 min. The samples were washed and fixed with PFA, and fluorescence quantification was analyzed by flow cytometry. Data are means of three independent experiments performed in triplicate ± SD. ((b), (d)) Determination of , , , and the number of EhBholoLfbp/cell was estimated by the method of Scatchard. In all cases, white and black squares indicate the nonvirulent amoebae and virulent amoebae, respectively. Data are means of three independent experiments performed in triplicate ± SD.
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