Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica

Table 1

Media used in this study.

Medium	Iron concentration (μM)	Source of iron	Reference
BI-S-33	100 ± 4.68	AFC, serum, and iron traces from reagents	Diamond et al., 1978 [15]
Low-iron	6.5 ± 2.61	Iron traces from reagents	Serrano-Luna et al., 1998 [10]
Low-iron plus serum	19 ± 4.35	Serum and iron traces from reagents	Serrano-Luna et al., 1998 [10]
Low-iron plus serum and B-holo-	100 ± 2.5, 115 ± 1.5, 125 ± 1.95 and 135 ± 1.85	Bovine Lf-iron, serum, and iron traces from reagents	[This work]

Iron concentration was measured by spectrophotometric method. Micro-Tec Laboratory, Mexico.
B-holo-Lf was added to obtain the iron concentrations indicated.