Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica

Table 2

Biochemical properties of binding/internalization of B-holo-Lf in E. histolytica.

	6°C			37°C
			EhBholoLfbp number			EhBholoLfbp number
	(M ± SD)^a	(μmol·min^−1± SD)^a	(sites/cell ± SD)^a	(M ± SD)^a	(μmol·min^−1± SD)^a	(sites/cell ± SD)^a
Nonvirulent amoebae	1.85 ± 0.002 × 10^−6	0.066 ± 0.0001	9.45 ± 0.093 × 10^5	2.5 ± 0.002 × 10^−6	0.4562 ± 0.00012	1.89 ± 0.00023 × 10^7
Virulent; amoebae	2.3 ± 0.0015 × 10^−6	1.659 ± 0.0013	6. 65 ± 0.011 × 10^6	2.48 ± 0.0012 × 10^−6	3.3 ± 0.0016	1.97 ± 0.00043 × 10^7
				5.2 ± 0.0027 × 10^−6	0.9 ± 0.00045

Three independent experiments were done by triplicate.
SD: standard deviation.