Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation

Figure 1

Expression of AP-2ε and the hypoxia marker angiopoietin-like 4 strongly correlate during chondrogenic differentiation. (a) 3D spheroid cultures of hMSC were cultured in chondrogenic medium for a period of 28 days and mRNA was isolated at days 1, 14, 21, and 28. Expression of both AP-2ε and ANGPTL4 significantly increased over the course of the experiment. (b) mRNA expression was analyzed in mesenchymal cells derived from the limb buds (LB) of E11.5 mouse embryos and in chondrocytes from the epiphyseal cartilage (EC) of 4-day-old new born mice, representing early and late stages of chondrogenic differentiation. In the latter, a significant upregulation of the AP-2ε as well as the Angptl4 mRNA level could be detected (data are given as means ± SEM; ns, not significant; ; ).

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