(a)
(b)
Figure 4: Effect of Cytochalasin D on PMNL apoptosis. We cultured PMNLs (1.5 × 106/mL) for 2.5 h at 37°C without or with 25 μg/mL sulfatides (St) or challenged with sulfatides-treated bacteria (St-Salm), and/or 10 μM Cyto D, as indicated. Shown are the mean ± SD (a) and representative flow cytometric spectra (b) of four independent experiments.