BioMed Research International / 2015 / Article / Fig 5

Research Article

Polymorphonuclear Leukocyte Apoptosis Is Accelerated by Sulfatides or Sulfatides-Treated Salmonella Typhimurium Bacteria

Figure 5

Phagocytosis of S. Typhimurium by PMNLs. We added PMNLs with plain bacteria (Salm), opsonized by normal serum (O-Salm) or treated by sulfatides (St-Salm) at a ratio of 10 bacteria/1 neutrophil. We assessed percentage of phagocyting cells by flow cytometry after 15 min of incubation of PMNLs with bacteria and removal of nonphagocytized bacteria. Shown are the mean ± SD (b) and representative flow cytometric spectra (a) of three independent experiments.