Study of Cytotoxic Effects of Benzonitrile Pesticides

Table 3

The inhibition coefficient (%) determined by the MTT test for Hep G2 and HEK293T cells for 25, 50, and 100 mg/L. The HEK293T cells were exposed for 24 h and Hep G2 cells for 48 h.

	(mg/L)	Hep G2	HEK293T
		(%)	(%)
BRX	100	91 ± 1	53 ± 2
	50	71 ± 1	25 ± 1
	25	28 ± 1	13 ± 3
BRAC	100	13 ± 3	7 ± 1
	50	15 ± 5	7 ± 1
	25	15 ± 7	10 ± 5
BRAM	100	17 ± 6	14 ± 1
	50	16 ± 3	12 ± 2
	25	8 ± 2	11 ± 4
DCB	100	9 ± 6	4 ± 4
	50	16 ± 8	9 ± 5
	25	21 ± 6	13 ± 7
DCBA	100	19 ± 4	10 ± 5
	50	21 ± 7	13 ± 5
	25	22 ± 3	9 ± 1
BAM	100	22 ± 5	12 ± 2
	50	20 ± 1	16 ± 2
	25	14 ± 4	14 ± 6
CHX	100	91 ± 1	39 ± 2
	50	74 ± 1	22 ± 4
	25	21 ± 3	19 ± 7
CHXAC	100	16 ± 6	18 ± 6
	50	17 ± 7	18 ± 7
	25	15 ± 4	19 ± 7
CHXAM	100	17 ± 7	22 ± 5
	50	19 ± 3	24 ± 4
	25	13 ± 2	16 ± 4
IOX	100	98 ± 1	61 ± 2
	50	87 ± 1	41 ± 6
	25	82 ± 3	22 ± 4
IOXAC	100	13 ± 7	14 ± 2
	50	13 ± 3	15 ± 2
	25	15 ± 6	18 ± 2
IOXAM	100	21 ± 4	25 ± 4
	50	19 ± 3	19 ± 6
	25	11 ± 2	18 ± 4

BRX: 3,5-dibromo-4-hydroxybenzonitrile, BRAC: 3,5-dibromo-4-hydroxybenzoic acid, BRAM: 3,5-dibromo-4-hydroxybenzamide, DCB: 2,6-dichlorobenzonitrile, DCBA: 2,6-dichlorobenzoic acid, BAM: 2,6-dichlorobenzamide, CHX: 3,5-dichloro-4-hydroxybenzonitrile, CHXAC: 3,5-dichloro-4-hydroxybenzoic acid, CHXAM: 3,5-dichloro-4-hydroxybenzamide, IOX: 3,5-diiodo-4-hydroxybenzonitrile, IOXAC: 3,5-diiodo-4-hydroxybenzoic acid, and IOXAM: 3,5-diiodo-4-hydroxybenzamide; SD: standard deviation of 3 parallel measurements; : the solubility of DCB in water is around 20 mg/L; therefore the inhibitions at concentrations 50 and 100 mg/L are only indicative; ±: standard deviation of 3 parallels.