Research Article
TOP2A Amplification and Overexpression in Hepatocellular Carcinoma Tissues
Table 1
Source, dilution, antigen retrieval method, and incubation time of different biomarkers.
| Marker | Source | Host | Dilution | Antigen retrieval | Incubation time |
| Her-2 | SP3 (Lab Vision, USA) | Rabbit | 1 : 400 | 10 mM citrate buffer pH 6.0, pressure cooker | 40 min, RT | Topo2α | Ki-SI (Lab Vision, USA) | Mouse | 1 : 400 | 10 mM citrate buffer pH 6.0, pressure cooker | 30 min, RT | P53 | Y5 (Lab Vision, USA) | Rabbit | 1 : 100 | 10 mM citrate buffer pH 6.0, pressure cooker | 30 min, RT | Ki-67 | SP6 (Lab Vision, USA) | Rabbit | 1 : 200 | 10 mM citrate buffer pH 6.0, pressure cooker | 30 min, RT |
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