BioMed Research International / 2015 / Article / Tab 1

Research Article

TOP2A Amplification and Overexpression in Hepatocellular Carcinoma Tissues

Table 1

Source, dilution, antigen retrieval method, and incubation time of different biomarkers.

MarkerSourceHostDilutionAntigen retrievalIncubation time

Her-2SP3 (Lab Vision, USA)Rabbit1 : 40010 mM citrate buffer pH 6.0, pressure cooker40 min, RT
Topo2αKi-SI (Lab Vision, USA)Mouse1 : 40010 mM citrate buffer pH 6.0, pressure cooker30 min, RT
P53Y5 (Lab Vision, USA)Rabbit1 : 10010 mM citrate buffer pH 6.0, pressure cooker30 min, RT
Ki-67SP6 (Lab Vision, USA)Rabbit1 : 20010 mM citrate buffer pH 6.0, pressure cooker30 min, RT