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BioMed Research International
Volume 2015 (2015), Article ID 382403, 8 pages
Research Article

Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia

1School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia
2CSIRO, Food and Nutrition Flagship, 671 Sneydes Road, Werribee, VIC 3030, Australia

Received 11 November 2014; Revised 15 February 2015; Accepted 23 February 2015

Academic Editor: Miguel Prieto

Copyright © 2015 Asanthi Perera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried ,  eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying , , , and ehxA and the other carrying alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.