DNaseI Protects against Paraquat-Induced Acute Lung Injury and Pulmonary Fibrosis Mediated by Mitochondrial DNA

Figure 1

PQ injures mitochondria and leads to mtDNA release. ((a)-(b)) The mitochondrial membrane potential decreased in A549 cells (changed from red to green) following a 24 h exposure to various doses of PQ. PQ reduced A549 cell viability (red line) in a dose- (c) and time-dependent manner ((d), PQ 600 μm) and was correlated with increased mtDNA release (black line) into the supernatant. (e) A549 cells were incubated with or without (control) 600 μm PQ for 12 h and then washed. Fresh medium was added, and the cells were cultured for 12 h. The supernatant mtDNA level was elevated among PQ-primed cells.

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