A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

Figure 2

(a) The figure is showing the HRM curve for LNA probes with complementary template and SM template, when different V/V ratios of formamide were added during hybridization; complementary-curve (I) and SM-curve (II): in buffer containing 10% formamide; complementary-curve (III) and SM-curve (IV): in buffer containing 20% formamide; complementary-curve (V) and SM-curve (VI): in buffer containing 30% formamide; complementary-curve (VII) and SM-curve (VIII): in buffer containing 40% formamide. (b) The figure is showing the first-order derivative curves of (a), and naming methods for these curves were the same as that of (a).

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