Table 10: Representative examples illustrating NGS strategies targeting GMO.

NGS strategiesNGS platformsTargetsTarget sizesReferences

Targeted sequencingHiSeq (Illumina)vip3Aa2 from MIR162150 bp to 2 Kbp[116]
PacBio RS (Pacific Biosciences)vip3Aa2 from MIR162150 bp to 2 Kbp[116]
454 system (Roche Applied Science)ssIIb157 bp[119]
454 system (Roche Applied Science)Bt11 gene324 bp[119]
454 system (Roche Applied Science)Bt176 gene206 bp[119]
454 system (Roche Applied Science)LEC118 bp[119]
454 system (Roche Applied Science)p35S/CTP4 171 bp[119]
454 system (Roche Applied Science)CP4-EPSPS498 bp[119]
454 system (Roche Applied Science)p35S195 bp[119]
454 system (Roche Applied Science)tNOS180 bp[119]

Whole genome sequencingHiSeq (Illumina)MON17903 soybean1115 Mbp[120]
HiSeq (Illumina)MON87704 soybean1115 Mbp[120]
HiSeq (Illumina)FP967 flax373 Mbp[121]
HiSeq (Illumina)LLRICE62 rice385 Mbp[122]
HiSeq (Illumina)TT51-1 rice385 Mbp[123]
HiSeq (Illumina)T1c-19 rice385 Mbp[123]
HiSeq (Illumina)Bt rice385 Mbp[124]

CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); CTP4 (chloroplast transit peptide 4 from the Arabidopsis thaliana epsps gene); LEC (lectin gene from soybean); p35S (promoter of the 35 S cauliflower mosaic virus); ssIIb (starch synthase IIb gene from maize); tNOS (terminator of the nopaline synthase gene); VIP3A (vegetative insecticidal protein 3A).