Table 4: Representative examples illustrating multiplex PCR CGE methods targeting GMO.

Multiplexing Methods Targets References Tetraplex Taxon-specific Zein and LEC [50] Element-specific p35S and tNOS Tetraplex Taxon-specific SAD1 [51] Element-specific Cry1Ac, p35S, and tNOS Pentaplex Taxon-specific ADH [52] Event-specific Bt11, GA21, MON810, and NK603 Hexaplex Taxon-specific acp1 [53] Event-specific Bollgard, Bollgard II, RR, 3006-210-23, and 281-24-231 Hexaplex Taxon-specific HMG [54, 55] Event-specific DAS-59122-7, LY038, MON88017, MIR604, and 3272 Octaplex Event-specific Bt11, Bt176, Huanong No. 1, GTS40-3-2, T25, MON88913, MON1445, and MIR604 [56] Octaplex Taxon-specific LEC and ssIIb   Element-specific pFMV and tNOS [56] Event-specific TC1507, MON531, NK603, and GA21   Octaplex Taxon-specific SAD1   Element-specific bar, chy, pAct, CP4-EPSPS, and Cry1Ab [56] Event-specific GT73 and OXY235   Nonaplex Taxon-specific HMG [57, 58] Event-specific T25, GA21, TC1507, MON863, MON810, NK603, Bt176, and Bt11

acp1 (acyl carrier protein 1 gene from cotton); ADH (alcohol dehydrogenase I gene from maize); bar (phosphinothricin-N-acetyltransferases gene from Streptomyces hygroscopicus); Chy (chymopapain gene from papaya); CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); HMG (major high-mobility group protein gene from maize); LEC (lectin gene from soybean); p35S (promoter of the 35 S cauliflower mosaic virus); pAct (promoter region of rice actin gene); pFMV (promoter of the figwort mosaic virus); SAD1 (stearoyl-acyl carrier protein desaturase gene from cotton); ssIIb (starch synthase IIb gene from maize); tNOS (terminator of the nopaline synthase gene); Zein (Zein gene from maize).