Table 5: Representative examples illustrating multiplex PCR microarray methods targeting GMO.

MultiplexingTechniquesMethodsTargetsReferences

DuplexDualChip GMOElement-specificp35S and tNOS[5961]
DuplexDualChip GMOConstruct-specificpNOS/nptII[5961]
Virus-specificCaMV
TriplexDualChip GMOElement-specificpat, Cry1A(b), and CP4-EPSPS[5961]
TriplexNAIMATaxon-specificIVR [62]
Element-specificp35S and tNOS
TriplexNAIMATaxon-specificIVR
Element-specificp35S[62]
Event-specificMON810
TetraplexDualChip GMOPlant-specificRBCL[5961]
Taxon-specificIVR, LEC, and CRU[63]
OctaplexMQDA-PCRTaxon-specificHMG
Element-specificp35S and tNOS
Event-specificBt176, Bt11, and MON810
OtherIPC
DecaplexPPLMDTaxon-specificSAD1, Zein, ACC, and LEC
Element-specificp35S, pFMV, and bar[64]
Event-specificMON1445, Bt176, and GTS40-3-2
DodecaplexMQDA-PCRTaxon-specificHMG [63]
Element-specificp35S, tNOS, and Amp
Event-specificBt176, Bt11, MON810, T25, GA21, CBH351, and DBT418
OtherIPC

ACC (acetyl-CoA-carboxylase gene from colza); Amp (ampicillin resistance gene); bar (phosphinothricin-N-acetyltransferases gene from Streptomyces hygroscopicus); CaMV (ORFIII from CaMV); CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); CRU (cruciferin gene from colza); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); HMG (major high-mobility group protein gene from maize); IPC (internal positive control); IVR (invertase gene from maize); LEC (lectin gene from soybean); nptII (neomycin phosphotransferase II gene); p35S (promoter of the 35 S cauliflower mosaic virus); pat (phosphinothricin-N-acetyltransferases gene from Streptomyces viridochromogenes); pFMV (promoter of the figwort mosaic virus); pNOS (promoter of the nopaline synthase gene); RBCL (ribulose-1,5-biphosphate carboxylase oxygenase); SAD1 (stearoyl-acyl carrier protein desaturase gene from cotton); tNOS (terminator of the nopaline synthase gene); Zein (Zein gene from maize).