Current and New Approaches in GMO Detection: Challenges and Solutions

Table 6

Representative examples illustrating Luminex strategies targeting GMO.

Multiplexing	Methods	Targets	References
Simplex	Element-specific	p35S and CP4-EPSPS	[65]
Triplex	Taxon-specific	Zein	[66]
	Event-specific	MIR604 and MON88017
Tetraplex	Event-specific	Bt176, MON810, NK603, and GA21	[66]
Tetraplex	Event-specific	Bt11, T25, MIR162, and MON89034	[66]
Pentaplex	Taxon-specific	SPS	[67]
	Element-specific	Cry1Ac, tNOS, p35S, and LS28
Hexaplex	Taxon-specific	SAD1
	Element-specific	Cry1Ac, Cry1F, and pat	[68]
	Event-specific	281-24-236 and 3006-210-23

CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); LS28 (choline kinase); p35S (promoter of the 35 S cauliflower mosaic virus) SAD1 (stearoyl-acyl carrier protein desaturase gene from cotton); SPS (sucrose phosphate synthase gene from rice); tNOS (terminator of the nopaline synthase gene); tORF23 (open reading frame 23 terminator from A. tumefaciens); Zein (Zein gene from maize).