Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells

Figure 2

In vivo consensus binding motif of SAFB1. (a) The frequency of pentamers surrounding SAFB1 crosslink sites was determined. Adenine represents 68% of the 20 pentamers that has the highest frequencies. (b) WebLogo showing base frequencies of each base at respective positions of the pentamer. SAFB1 binds to adenine-rich motifs. (c) The frequency of each base relative to its position within the pentamer was summarised in this table. The highest frequency of adenine was observed at base position 5; thymine was excluded at base position 4 of the consensus binding motif. This consensus binding motif was predicted from iCLIP cDNA libraries; therefore the uracil base is referred to as thymine in these sequences.

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