Research Article

The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone

Figure 3

The check of apoptotic marker caspase-3. (a)–(f) Localization of the apoptotic marker active caspase-3 using immunohistochemistry. The apoptotic molecular marker, active caspase-3, was mainly localized in the granulosa cell and oocyte and specifically in the primary follicle, secondary follicle, and antral follicle. (a) CG. (b) NG-FSH. (c) OG-FSH. (d) EG-FSH. (e) LG-FSH. (f) Negative control. (g) Caspase-3 protein expression. Caspase-3 protein expression in the CG, OG-FSH, EG-FSH, LG-FSH, and NG-FSH groups. The different letters indicate significant differences; the same letters indicate no differences.