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BioMed Research International
Volume 2015, Article ID 397264, 13 pages
Research Article

The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone

1Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetics in Ningxia, Department of Histology and Embryology, Ningxia Medical University, Yinchuan 750004, China
2Department of Human Anatomy, Inner Mongolia Medical University, Hohhot 010010, China

Received 17 June 2015; Revised 23 August 2015; Accepted 30 August 2015

Academic Editor: Mohammed Rachidi

Copyright © 2015 Yanzhou Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Response: Checked and revised.

Supplementary 1: The preparation of medium including of basic medium, cultural solution, vitrified solution and warming solution.

Supplementary 2: The process of ovarian transplantation

  • a: The ovaries were heterotopically transplanted by the kidney capsule.

  • b: Ovaries were orthotopically transplanted into ovarian capsule.

Supplementary 3: The Ovaries from C57BL/6J strain mice (black) were orthotopically transplanted into C57BL/6J-Tyrc-2JJ albino strain mice (white), and then were mated with proven males of the same strain to induce natural pregnancy and birth.

  1. Supplementary Materials