The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone

Table 1

Primer sequences used for real-time quantitative PCR.

Target gene	GenBank accession number	Primer sequence	Product size (bp)	Annealing temperature (°C)
GAPDH	XM_001476707.3	F: 5′-GGCATTGTGGAAGGGCTC-3′; R: 5′-GACCTTGCCCACAGCCTT-3′	156	60
Cx43	NM_010288.3	F: 5′-CGTCCCACGGAGAAAACCAT-3′; R: 5′-CGGTGGTGGCGTGGTAAG-3′	151	60
Cx37	NM_008120.3	F: 5′-AGCGGTTGCGGCAGAAAG-3′; R: 5′-CCCACGAATCCGAAGACGAC-3′	137	60
VEGF	NM_001025257.3	F: 5′-GTAACGATGAAGCCCTGGAGT-3′; R: 5′-TGTTCTGTCTTTCTTTGGTCTGC-3′	152	60