The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro

Figure 2

QRT-PCR validation of RNA-Seq results. The relative gene expression of selected genes shows altered expression upon hyphae development as compared to yeast-like growth. Black and open bars represent control hyphae (C. albicans cells grown in CKM) and hyphae developed in the presence of PK E6/E7 vaginal epithelial cells, respectively. The ratio of each mRNA relative to the 18S rRNA was calculated using the method. Data are representative of 3 or more independent experiments and are presented as mean ± standard deviation (SD). The significance of differences between sets of data was determined by two-sample -test; . For gene names, please see Table S1 (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/398045).