Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries

Figure 1

Reactivity of purified IgG antibodies against L. infantum chagasi antigen (LiPA). 10 μg of affinity-purified IgGs from CVL dogs (●) and negative dogs (■) was added to each well (from 10 μg to 0.625 μg/well) of microtiter plates coated with 50 μg/mL LiPA. The reaction was detected using a peroxidase conjugated anti-dog IgG antibody (1 : 2000). Absorbance values at 492 nm were means of duplicates.