Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries

Figure 2

(a) Enrichment of phage binding after three rounds of panning. 50 μL of a 10¹⁰ transduction unit phage suspension collected after each round of panning was added to wells of microtiter plates coated with 5 μg/mL anti-LiPA IgG. Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are means of duplicates. (b) Reactivity of individual clones isolated after panning 3. 50 μL of a 10¹⁰ transduction unit phage suspension isolated after the third round of panning was added to each well of microtiter plates coated with 1 μg/well of anti-L. infantum chagasi IgGs (●) and anti-T. cruzi IgGs (■). Phages were detected using a peroxidase conjugated anti-M13 antibody (1 : 3000). Values of absorbance at 492 nm are means of duplicates. (c) The aminoacid sequences of the three selected peptides.

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