Immunodiagnosis of Canine Visceral Leishmaniasis
Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries
Table 2
Kappa index (κ) between paired results of diagnostic tests using peptides, L. infantum chagasi antigen, and EIE-LVC kit.
Diagnostic test
EIE-LVC#
Ag*
P
N
T
P
N
T
Pep-5*
P
36
2
38
36
2
38
N
21
48
69
9
60
69
T
57
50
107
45
62
107
κ index (95% CI)
0.578 (0.435–0.721), moderate
0.784 (0.665–0.904), good
Pep-6*
P
37
3
40
40
0
40
N
20
47
67
14
53
67
T
57
50
107
54
53
107
κ index (95% CI)
0.557 (0.407–0.708), moderate
0.739 (0.616–0.862), good
Pep-11*
P
36
2
38
36
2
38
N
21
48
69
9
60
69
T
57
50
107
45
62
107
κ index (95% CI)
0.578 (0.435–0.721), moderate
0.784 (0.665–0.904), good
Pep-Mix*
P
37
3
40
40
0
40
N
20
47
67
14
53
67
T
57
50
107
54
53
107
κ index (95% CI)
0.557 (0.407–0.708), moderate
0.739 (0.616–0.862), good
Ag*
P
41
12
53
N
16
38
54
T
57
50
107
κ index (95% CI)
0.477 (0.311–0.643), moderate
Samples from healthy dogs and dogs with canine visceral leishmaniasis, T. cruzi, E. canis, or B. canis. *Cut-off obtained by ROC curve.
#Cut-off obtained according to the manufacturer. P: positive; N: negative; T: total; CI: confidence interval.
