Experience of Preimplantation Genetic Diagnosis for Hemophilia at the University Hospital Virgen Del Rocío in Spain: Technical and Clinical Overview
Table 1
Clinical data for PGD of hemophilia at HUVR.
No embryos obtained
Embryo sexing by FISH
Embryo sexing by PCR
Embryo sexing in general
Specific PCR method for HA
Total
Number of couples treated
1
14
2
16
18
34
Maternal age
40
31.0 ± 4.9
30.5 ± 3.5
31.8 ± 2.9
31.6 ± 4.1
Number of cycles performed
2
25
4
29
29
60
Number of cycles performed per couple
2
1.9 ± 0.8
2.0 ± 1.4
1.9 ± 0.8
1.7 ± 0.8
1.8 ± 0.8
Number of mature oocytes submitted to ICSI
7
317 (280 HA + 37 HB)
43 (HB)
360 (280 HA + 80 HB)
408
775
Number of mature oocytes submitted to ICSI per cycle
3.5 ± 4.9
12.7 ± 4.7
10.8 ± 5.1
12.4 ± 4.7
13.6 ± 7.3
13.0 ± 6.2
Number of oocytes fertilized
0
217 (187 HA + 30 HB)
27 (HB)
244 (187 HA + 57 HB)
264
508
% of oocytes fertilized
—
68.5%
62.8%
67.8%
64.7%
65.6%
Number of oocytes fertilized per cycle
—
8.7 ± 3.5
6.8 ± 3.4
8.4 ± 3.5
8.8 ± 5.6
8.6 ± 4.7
Number of embryos analyzed
—
155 (132 HA + 23 HB)
23 (HB)
178 (132 HA + 46 HB)
205
383
% of embryos analyzed
—
71.4%
85.2%
73.0%
77.7%
75.4%
Number of embryos analyzed per cycle
—
6.2 ± 2.3
5.8 ± 2.5
6.1 ± 2.3
6.8 ± 4.5
6.5 ± 3.6
Number of informative embryos
—
124 (104 HA + 20 HB)
19
143 (104 HA + 39 HB)
180
323
% of informative embryos
—
80.0%
82.6%
80.3%
87.8%
84.3%
Number of transfers
—
20 (17 HA + 3 HB)
3 (HB)
23 (17 HA + 6 HB)
26
49
% of transfers
—
80.0%
75.0%
79.3%
89.7%
81.7%
Number of embryos transferred
—
29 (24 HA + 5 HB)
6
35 (24 HA + 11 HB)
48
83
Number of biochemical pregnancies
—
6 (6 HA)
1 (HB)
7 (6 HA + 1 HB)
12
19
Number of clinical pregnancies
—
3 (3 HA)
1 (HB)
4 (3 HA + 1 HB)
7
11
% of clinical pregnancies per cycle
—
12.0%
25.0%
13.8%
24.1%
18.3%
% of clinical pregnancies per transfer
—
15.0%
33.3%
17.4%
26.9%
22.5%
Implantation rate
—
10.3%
33.3%
14.3%
16.7%
15.7%
Number of pregnancies went to term
—
2
1
3
7
10
Number of babies born
—
2
2
4
8
12
Live birth rate per cycle
—
8.0%
25.0%
10.3%
24.1%
16.7%
One of the couples was submitted to two PGD cycles using FISH for embryo sexing and to a third cycle using the specific multiplex PCR method for hemophilia A.
