Research Article
A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A
Table 1
Primers for the simultaneous detection of Ancylostoma ceylanicum, A. caninum, and Giardia lamblia assemblage A by multiplex PCR.
| | Target parasite | Primer name | Amplicon size (bp) | Sequences (5′-3′) |
| | A. ceylanicum | A.cey-F | 191 | CCCCGTTACAGCCCTACGAG | | A.cey-R | TCCTGACAGACAAGTGCCGAACT |
| G. lamblia
assemblage A | 5C1-P21-F | 303 | ATGCTAGCCGTAGTTAATAAGG | | 5C1-P21-R | ACCGGCCTTATCTACCAGC |
| | A. caninum | A.can-F | 427 | AGCATTAGGCTAACGCCCGA | | A.can-R | AACGAGTTTGCTGTCATTCAGTCC |
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