Research Article
A Multiplex PCR for Simultaneous Detection of Three Zoonotic Parasites Ancylostoma ceylanicum, A. caninum, and Giardia lamblia Assemblage A
Table 2
Optimization results of simplex PCR.
| | Parasite | Primers concentration (μM) | Mg2+ concentration (mM) | Annealing temperature (°C) |
| | A. ceylanicum | 0.4–1.2 | 1.0–4.0 | 51–65 | | A. caninum | 0.4–1.2 | 1.0–4.0 | 51–65 | G. lamblia
assemblage A | 0.2–1.2 | 1.0–4.0 | 51–65 |
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