Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery

Figure 3

Copolymer P2 forms complexes with siRNA at different molar ratios and the binding stability was tested by gel electrophoresis. Loading buffer 1 contains glycerol, HEPES buffer (pH 7.3), and EDTA. Loading buffer 2 has two more components: Triton X-100 (1% v/v) and potassium polyvinyl sulfate (PVSK) to disrupt the polymer/siRNA complex.