Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery

Figure 5

Copolymer P1 (0.5 μM) was transfected to the A549-luc cells for 72 h. (a) Fluorescence was detected by a fluorescent microscope, (b) determination of cellular uptake by a Tali image-based cytometer, (c) monitoring the localization of P1 by three-dimensional stacks generated from a series of confocal plane images with 1.0 μm steps.

(a)

(b)

(c)