Impairment of Neutrophil Migration to Remote Inflammatory Site during Lung Histoplasmosis

Figure 4

PMN recruitment to air pouch induced by PAF is dependent on endogenous leukotrienes. 5-LO^−/− mice were inoculated with PBS or infected with Hc (i.t., 3 × 10⁶ yeast). After 2 days, LTB₄ (0.1 μg), PAF (1 μg), or PBS (1 mL) were injected into air pouch, and, 4 h after, PMN were recovered and the counts were determined as described in Materials and Methods. Data are the mean ± SEM of = 4-5. versus vehicle (noninfected mice); versus noninfected (LTB₄ into air pouch).