mir-21 Overexpressing Mesenchymal Stem Cells Accelerate Fracture Healing in a Rat Closed Femur Fracture Model

Figure 1

mir-21 promoted osteogenesis of rBMSCs in vitro. (a) rBMSCs were treated with mir-21 in osteogenic induction medium for 14 days. Mineralized nodules were stained by Alizarin Red S. (b) Alizarin red S concentrations were quantified by absorbance measurement at 550 nM. (c-d) rBMSCs were treated with mir-21 in osteogenic induction medium for 3 (c) and 7 days (d). Osteogenesis-related gene expressions were detected by q-PCR. (e) mir-21-5p expression level was detected 3 days after osteogenic induction. (f) SOX-2, the potential target of mir-21, was also detected 3 days after osteogenic induction. β-actin was used as internal control. The experiments were repeated three times. , compared with control.

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