DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

<div>Analysis of the products was performed by 8% acrylamide gel electrophoresis. PCR products of <i>UBP4 </i><sup>′′</sup><i>b</i>: M-marker (bp) GeneRuler DNA Ladder Mix (Fermentas Life Sciences), lane 1: <i>UBP4 </i><sup>′′</sup><i>b</i> gene (543 bp <i>UBP4 </i><sup>′′</sup><i>b</i> length and 26 bp for restriction enzymes sequence = 569 bp). PCR reaction with Biotools DNA polymerase and 23 cycles.</div>

BioMed Research International

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Figure 5

Figure 5: DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature 