Successive Intramuscular Boosting with IFN-Alpha Protects Mycobacterium bovis BCG-Vaccinated Mice against M. lepraemurium Infection

Figure 1

Schematic representation of the prime-boost protocol designed for the study. Adult BALB/c mice were s.c. primed with PBS or 5 × 10⁵ CFUs BCG. Four weeks later, in consecutive days (30, 31, and 32 days), the mice of each group received PBS or 100 UI IFN-α i.m. Mice were rested for 10 weeks and then challenged by intradermal route with 2 × 10⁶ M. lepraemurium. Eight weeks after challenge, mice were sacrificed and skin lesion development was measured.