Successive Intramuscular Boosting with IFN-Alpha Protects Mycobacterium bovis BCG-Vaccinated Mice against M. lepraemurium Infection

Table 1

Antibody response in BALB/c mice with the BCG-priming/IFN-alpha boost after M. lepraemurium challenge.

Vaccination group	IgM	IgG	IgG1	IgG2a	IgG2b	IgG3
Control	0.87 ± 0.09	1.10 ± 0.12	1.70 ± 0.34	1.10 ± 0.22	1.30 ± 0.18	0.87 ± 0.15
BCG	0.90 ± 0.13	1.10 ± 0.18	1.80 ± 0.07	1.80 ± 0.20	1.60 ± 0.31	0.90 ± 0.13
IFN-α	0.98 ± 0.17	1.50 ± 0.21	2.20 ± 0.22	1.70 ± 0.17	1.50 ± 0.16	1.14 ± 0.05
BCG-IFN-α	1.20 ± 0.55	1.34 ± 0.69	1.95 ± 0.68	1.90 ± 0.77	1.82 ± 0.56	1.30 ± 0.55

Eight weeks after infection with MLM, BCG-primed/i.m. IFN-alpha boosted mice were sacrificed. Levels of antiglycolipid-1 isotype and all IgG subclasses Abs were measured in serum and determined by immunoenzymatic assay ELISA. Values are expressed as OD 450 nm and represent median ± SEM of duplicates.