Successive Intramuscular Boosting with IFN-Alpha Protects Mycobacterium bovis BCG-Vaccinated Mice against M. lepraemurium Infection

Table 2

Cytokine profile in BALB/c mice with BCG-priming/IFN-alpha boost after M. lepraemurium challenge.

Vaccination group	IFN-γ	IL-4	IL-17	TNF-α	IL-10
SERUM
Control	912 ± 102	1360 ± 406	762 ± 58	511 ± 147	1826 ± 318
BCG	1274 ± 283	1805 ± 149	1062 ± 222	1675 ± 670	2060 ± 140
IFN-α	1076 ± 233	1683 ± 211	999 ± 177	1485 ± 560	2397 ± 514
BCG-IFN-α	1460 ± 187	1870 ± 451	1128 ± 260	1815 ± 467	2477 ± 169

Eight weeks after infection with MLM, BCG-primed/IFN-alpha boosted mice were sacrificed. Levels of cytokines were measured in serum and determined by immunoenzymatic assay ELISA (PeproTech, Inc). Values are expressed to pg/mL and represent median ± SEM of duplicates.