Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway

Figure 1

Black rice anthocyanins (BRACs) extract inhibits migration and invasion of human epidermal growth factor receptor 2 (HER2⁺) breast cancer MDA-MB-453 cell line. MCF-10A and MDA-MB-453 cells were exposed to BRACs (0 or 200 μg/mL) for 24 h. (a) Cell migration was determined using wound healing migration assay (×20). (b) MCF-10A and MDA-MB-453 cells were plated in upper compartments of Matrigel invasion chambers and exposed to BRACs (0 or 200 μg/mL). Then, invasive potential of treated cells was evaluated microscopically. (c) Migrating cell numbers were counted using a microscope. . (d) Number of invading cells was determined by counting using a microscope. . Data are mean ± SEM of three independent experiments.

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