Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway

Figure 10

Effects of black rice anthocyanins (BRACs) extract on MMP2 and MMP9. (a) MDA-MB-453 cells were treated with BRACs (0 or 200 μg/mL) for 24 h. Cells were harvested for analysis of MMP2 and MMP9 expression using immunoblotting. Expression of β-actin served as a loading control. (b) MDA-MB-453 cells were treated with BRACs (0 or 200 μg/mL) for 24 h. Cell lysates were collected and immunoprecipitated with anti-HER2 antibody and then immunoblotted with antibodies against MMP2, MMP9, and HER2. (c) MDA-MB-453 cells were treated with BRACs (0 or 200 μg/mL) for 24 h. Cell lysates were collected and immunoprecipitated with an anti-ERK antibody and then immunoblotted with antibodies against MMP2, MMP9, and ERK. (d) MDA-MB-453 cells were treated with BRACs (0 or 200 μg/mL) for 24 h. Cell lysates were collected and immunoprecipitated with an anti-JNK antibody and then immunoblotted with antibodies against MMP2, MMP9, and JNK.

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