Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway

Figure 2

Black rice anthocyanins (BRACs) extract and rapidly RAF/MEK/JNK inhibitors decrease migration and invasion of MDA-MB-453 cells. (a) MDA-MB-453 cells were exposed to BRACs (0 or 200 μg/mL) with or without RAF/MAPK inhibitors ZM33672 (70 nM), U0126 (10 μM), or SP600125 (50 nM) for 24 h. Cell migration was determined using wound healing migration assay and numbers of migratory cells was determined by counting using a microscope; versus control and versus BRACs groups. (b) MDA-MB-453 cells were plated in upper compartments of Matrigel invasion chambers and exposed to BRACs (0 or 200 μg/mL) with or without RAF/MAPK inhibitors ZM33672 (70 nM), U0126 (10 μM), or SP600125 (50 nM). versus control; versusBRACs groups. Data are mean ± SEM of three independent experiments.

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