Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway

Figure 3

Black rice anthocyanins (BRACs) extract and small interfering RNAs (siRNA) blocked migration and invasion of MDA-MB-453 human epidermal growth receptor 2 (HER2⁺) breast cancer cells. (a) MDA-MB-453 cells were exposed to BRACs (0 or 200 μg/mL) with or without transfection with raf-, mek-, or jnk-siRNAs for 24 h. Cell migration was determined using wound healing migration assay and number of migratory cell was counted using a microscope. versus control and versus BRACs groups. (b) MDA-MB-453 cells were plated in the upper compartments of Matrigel invasion chambers and exposed to BRACs (0 or 200 μg/mL) with or without transfection with raf-, mek-, or jnk-siRNAs. versus control and versus BRACs groups. Data are mean ± SEM of three independent experiments.

(a)

(b)