Research Article

Iron-Binding Protein Degradation by Cysteine Proteases of Naegleria fowleri

Figure 2

Zymography assay in 10% PAGE copolymerized with 0.1% (w/v) holo-bLf. (a) CM was evaluated at different pHs at 37°C: molecular weight marker (lane 1), pH 5 (lane 2), pH 7 (lane 3), and pH 9 (lane 4). (b) Effects of protease inhibitors evaluated at pH 7 37°C: pHMB (lane 1), PMSF (lane 2), and aprotinin (lane 3).
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(b)