Iron-Binding Protein Degradation by Cysteine Proteases of Naegleria fowleri

Figure 6

Zymography assay in 10% PAGE copolymerized with 0.1% (w/v) hHb. (a) CM was evaluated at different pHs at 37°C. Molecular weight marker (lane 1), pH 5 (lane 2), pH 7 (lane 3), and pH 9 (lane 4). (b) Effects of protease inhibitors: pHMB (lane 1), PMSF (lane 2), and aprotinin (lane 3).

(a)

(b)