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BioMed Research International
Volume 2015, Article ID 416712, 8 pages
http://dx.doi.org/10.1155/2015/416712
Research Article

Iron-Binding Protein Degradation by Cysteine Proteases of Naegleria fowleri

1Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Avenida IPN 2508, 07360 Mexico City, Mexico
2Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute, Avenida IPN 2508, 07360 Mexico City, Mexico
3Faculty of Professional Studies, Autonomous University of Mexico, Campus Cuautitlán, Km 2.5 Carretera Cuautitlán-Teoloyucan, 54714 Cuautitlán Izcalli, Mexico

Received 22 September 2014; Revised 17 December 2014; Accepted 19 December 2014

Academic Editor: Mehdi Chenik

Copyright © 2015 Moisés Martínez-Castillo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The results of the supplementary figures showed the degradation activities of total crude extract (TCEs) of N. fowleri. We analyzed the degradation pattern of different iron-binding proteins such as human and bovine holo-lactoferrin, human holo-transferrin, equine ferritin, and human hemoglobin at different pHs and at 37°C. Also we evaluated the type of proteases using different proteases inhibitors.

  1. Supplementary Material