Transgenic Carrot Expressing Fusion Protein Comprising M. tuberculosis Antigens Induces Immune Response in Mice

Figure 1

Schemes for assembly of the fusion gene (a) and for the T region in the binary plasmid pBI121 carrying sequence of the fusion gene cfp10-esat6-dIFN (b). cfp10, nucleotide sequence of the M. tuberculosis cfp10 gene; esat6, sequence of the M. tuberculosis esat6 gene; dIFN, sequence of the human deltaferon gene; cfp10-esat6-dIFN, sequence of the fusion gene; nptII, E. coli neomycin phosphotransferase II gene; and uidA, sequence of the E. coli β-glucuronidase gene. RB and LB are the repeats flanking the plasmid T region; pNOS and tNOS, nopaline synthase gene of the A. tumefaciens Ti plasmid promoter and terminator correspondingly; CaMV35S, cauliflower mosaic virus 35S RNA gene promoter. Link, gly–ala–gly hinge joints; and up1, up2, up3, lo1, lo2, and lo3 denote the positions of the corresponding primers. Arrows show the sites for BamHI and XbaI restriction endonucleases. The lengths of the corresponding gene sequences in base pairs are in parentheses.

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