A Novel Microbisporicin Producer Identified by Early Dereplication during Lantibiotic Screening

Figure 3

Phylogenetic tree derived from the 16S rRNA gene sequences of Actinoallomurus species and related actinomycetes belonging to the Thermomonosporaceae family. Sequences from actagardine, planosporicin, and microbisporicin actinomycete producers were also included. For the construction of the phylogenetic tree, selected sequences were aligned with Clustal-Omega (from the EMBL-EBI site) and analyzed with BioEdit [30]. Distance matrices were calculated with MEGA5.2, using the Maximum Likelihood method implemented in the program and the method of Jukes and Cantor. Trees were inferred using the Nearest-Neighbor-Interchange (NNI) heuristic method and making the initial tree with both Neighbour Joining and BioNJ, and selecting the superior tree (all methods are included in the MEGA package). All analyses were performed on a bootstrapped data set containing 500 replicates.