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BioMed Research International
Volume 2015, Article ID 419549, 11 pages
http://dx.doi.org/10.1155/2015/419549
Research Article

Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production in Mycobacterium colombiense Strains

1Chemistry Department, Faculty of Sciences, Universidad Nacional de Colombia, Carrera 30, No. 45-03, Ciudad Universitaria, Bogotá, Colombia
2Computational Biology Branch, NCBI, NLM, NIH, Bethesda, MD 20894-6075, USA
3Pan American Bioinformatics Institute, Santa Marta, Magdalena, Colombia

Received 15 December 2014; Revised 15 January 2015; Accepted 16 January 2015

Academic Editor: Mehdi Mirsaeidi

Copyright © 2015 Milena Maya-Hoyos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mycobacterium colombiense is a novel member of the Mycobacterium avium complex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features of M. colombiense strains remain unknown. In the present study, we demonstrated that M. colombiense strains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates. Thin-layer chromatography experiments showed that M. colombiense strains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) of M. avium. Conversely, we observed a natural rough variant of M. colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis in M. colombiense CECT 3035. RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis.